sl-a 1· buffer Search Results


d d w a b a b a sic a n d tr a n sla tio n a l a t cipitated with saturated ammonium sulphate solubilized in 0 1 mol l borate phosphate buffer vol vol  (Thermo Fisher)
97
Thermo Fisher d d w a b a b a sic a n d tr a n sla tio n a l a t cipitated with saturated ammonium sulphate solubilized in 0 1 mol l borate phosphate buffer vol vol
D D W A B A B A Sic A N D Tr A N Sla Tio N A L A T Cipitated With Saturated Ammonium Sulphate Solubilized In 0 1 Mol L Borate Phosphate Buffer Vol Vol, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sl-a+1%C2%B7+buffer/Phosphate/pm22750506-45-4-51
Average 97 stars, based on 1 article reviews
d d w a b a b a sic a n d tr a n sla tio n a l a t cipitated with saturated ammonium sulphate solubilized in 0 1 mol l borate phosphate buffer vol vol - by Bioz Stars, 2026-09
97/100 stars
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media reagent quantity supplier sodium lactate agar sla double distilled water 1 l tryptone 10  (Thermo Fisher)
99
Thermo Fisher media reagent quantity supplier sodium lactate agar sla double distilled water 1 l tryptone 10
Media Reagent Quantity Supplier Sodium Lactate Agar Sla Double Distilled Water 1 L Tryptone 10, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sl-a+1%C2%B7+buffer/AGAR/us07427397-1678-0-16
Average 99 stars, based on 1 article reviews
media reagent quantity supplier sodium lactate agar sla double distilled water 1 l tryptone 10 - by Bioz Stars, 2026-09
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serum monoclonal mouse anti sla class i antibody  (ATCC)
93
ATCC serum monoclonal mouse anti sla class i antibody
Effects of <t>anti-SLA</t> I antibody on PCA grafts. Representative H&E and immunohistochemistry staining. A, PCA recovered after infusion of 200 μg of isotype control antibody without evidence of thrombosis. B, PCA recovered after infusion of 100 μg anti-SLA I. There is widespread thrombosis (asterisk) across the lumen of the graft. C, A high magnification view of Panel B (boxed region) shows attached, but swollen, retracted endothelial cells (ECs) (open arrows and higher magnification insert). Closed arrow shows a group of putative delaminated ECs. D–F, Immunostaining of anti-mouse IgG (D), C4d (E), and F4/80 (F). The asterisk indicates the site of thrombosis. Anti-mouse IgG staining in panel D shows extensive pig-specific mouse anti-SLA I antibody deposition in pig graft, on cells within the lumen and on cells overlaying the thrombus (open arrows). The insert in panel D is a negative control showing anti-mouse IgG staining in an implanted PCA not challenged with anti-SLA I antibody. Open arrows in panel F indicate F4/80-positive mouse macrophages interspersed with pig ECs. Closed arrow in panel F indicates a cluster of mouse monocytes. These cells are also evident in panel G. G, In situ hybridization showing the distribution of mouse (red) and pig (green) cells. Note clusters of mouse monocytes in the lumen (white arrow) and interdigitating mouse and pig cells along the vessel wall. H shows the mean ± standard deviation of the percent area of thrombosis after adoptive transfer of anti-pig SLA (0–200 μg) and isotype control (200 μg) antibody. The asterisk indicates that the level of thrombosis was significantly higher compared to the isotype control (P < 0.05). The extent of thrombosis increased in a dose-dependent manner (Spearman correlation coefficient = 0.9493, P value < 0.0001).
Serum Monoclonal Mouse Anti Sla Class I Antibody, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sl-a+1%C2%B7+buffer/7-34-1/pmc10022689-115-2-13
Average 93 stars, based on 1 article reviews
serum monoclonal mouse anti sla class i antibody - by Bioz Stars, 2026-09
93/100 stars
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Image Search Results


Effects of anti-SLA I antibody on PCA grafts. Representative H&E and immunohistochemistry staining. A, PCA recovered after infusion of 200 μg of isotype control antibody without evidence of thrombosis. B, PCA recovered after infusion of 100 μg anti-SLA I. There is widespread thrombosis (asterisk) across the lumen of the graft. C, A high magnification view of Panel B (boxed region) shows attached, but swollen, retracted endothelial cells (ECs) (open arrows and higher magnification insert). Closed arrow shows a group of putative delaminated ECs. D–F, Immunostaining of anti-mouse IgG (D), C4d (E), and F4/80 (F). The asterisk indicates the site of thrombosis. Anti-mouse IgG staining in panel D shows extensive pig-specific mouse anti-SLA I antibody deposition in pig graft, on cells within the lumen and on cells overlaying the thrombus (open arrows). The insert in panel D is a negative control showing anti-mouse IgG staining in an implanted PCA not challenged with anti-SLA I antibody. Open arrows in panel F indicate F4/80-positive mouse macrophages interspersed with pig ECs. Closed arrow in panel F indicates a cluster of mouse monocytes. These cells are also evident in panel G. G, In situ hybridization showing the distribution of mouse (red) and pig (green) cells. Note clusters of mouse monocytes in the lumen (white arrow) and interdigitating mouse and pig cells along the vessel wall. H shows the mean ± standard deviation of the percent area of thrombosis after adoptive transfer of anti-pig SLA (0–200 μg) and isotype control (200 μg) antibody. The asterisk indicates that the level of thrombosis was significantly higher compared to the isotype control (P < 0.05). The extent of thrombosis increased in a dose-dependent manner (Spearman correlation coefficient = 0.9493, P value < 0.0001).

Journal: Xenotransplantation

Article Title: A pig-to-mouse coronary artery transplantation model for investigating the pathogenicity of anti-pig antibody

doi: 10.1111/xen.12198

Figure Lengend Snippet: Effects of anti-SLA I antibody on PCA grafts. Representative H&E and immunohistochemistry staining. A, PCA recovered after infusion of 200 μg of isotype control antibody without evidence of thrombosis. B, PCA recovered after infusion of 100 μg anti-SLA I. There is widespread thrombosis (asterisk) across the lumen of the graft. C, A high magnification view of Panel B (boxed region) shows attached, but swollen, retracted endothelial cells (ECs) (open arrows and higher magnification insert). Closed arrow shows a group of putative delaminated ECs. D–F, Immunostaining of anti-mouse IgG (D), C4d (E), and F4/80 (F). The asterisk indicates the site of thrombosis. Anti-mouse IgG staining in panel D shows extensive pig-specific mouse anti-SLA I antibody deposition in pig graft, on cells within the lumen and on cells overlaying the thrombus (open arrows). The insert in panel D is a negative control showing anti-mouse IgG staining in an implanted PCA not challenged with anti-SLA I antibody. Open arrows in panel F indicate F4/80-positive mouse macrophages interspersed with pig ECs. Closed arrow in panel F indicates a cluster of mouse monocytes. These cells are also evident in panel G. G, In situ hybridization showing the distribution of mouse (red) and pig (green) cells. Note clusters of mouse monocytes in the lumen (white arrow) and interdigitating mouse and pig cells along the vessel wall. H shows the mean ± standard deviation of the percent area of thrombosis after adoptive transfer of anti-pig SLA (0–200 μg) and isotype control (200 μg) antibody. The asterisk indicates that the level of thrombosis was significantly higher compared to the isotype control (P < 0.05). The extent of thrombosis increased in a dose-dependent manner (Spearman correlation coefficient = 0.9493, P value < 0.0001).

Article Snippet: Antibody and serum Monoclonal mouse anti-SLA class I antibody was produced from CRL-1945 (American Type Culture Collection, CRL-1945) grown in MAB Quantum Yield media (BD Bioscience, San Jose, CA) with 5% immunoglobulin-free FCS (Hyclone, Logan, UT, USA) and purified by protein G chromatography.

Techniques: Immunohistochemistry, Staining, Control, Immunostaining, Negative Control, In Situ Hybridization, Standard Deviation, Adoptive Transfer Assay